cd40 knockout cd40 ” mice  (Jackson Laboratory)

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: MDSC were isolated from the spleens of WT C57BL/6 mice without tumor inoculation (tumor-free; n = 5) or from the spleens and tumors of mice with tumors (diameter = 1 cm; n = 5) grown from subcutaneously injected MFC, RM-1 or LLC cells. CD40 + % MDSC were analyzed using flow cytometry after staining isolated MDSC with PE-conjugated anti-CD40 antibody (CD40 − PE; blue line) or PE-conjugated isotype-matched IgG control antibody (red line). a. Representative flow cytometry images showing minimal CD40 + cell detection in tumor-free mouse spleen, and a dramatic increase in CD40 + % MDSC after tumor formation from all three different types of cancer cells. The highest CD40 + % levels were detected in tumor tissues. b. CD40 + % quantification in different groups of mice. * p < 0.05 and ** p < 0.01, compared to the corresponding tumor tissues.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Isolation, Injection, Flow Cytometry, Staining, Control

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Single cells dissociated from MFC tumors were stained with fluorophore-conjugated anti-CD11b, anti-Gr-1 and anti-CD40 antibodies. The gating strategy for FACS as well as the pre- and post-sorting percentages of CD40 high and CD40 low MDSC are shown.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Staining

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Gene expression profiles of CD40 high MDSC and CD40 low MDSC were examined by microarray analysis. a. Scatter plot of microarray data showing genes that were more than two-fold upregulated (red), less than two fold changed (black) or downregulated by more than two fold (green) in CD40 high compared with CD40 low MDSC. b. Heat map representation of microarray data on genes of interest. Expression levels are indicated on a color scale where red represents higher expression and green lower expression. c. qRT-PCR analysis of eight genes comparing CD40 high and CD40 low MDSC expression levels. The expression level of each target gene relative to an internal control (β-actin) was determined using the 2 −ΔΔCT method. The fold change between the two cell groups is presented.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Gene Expression, Microarray, Expressing, Quantitative RT-PCR, Control

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Flow cytometry analysis of the percentage of CD11b + Gr-1 + MDSC (MDSC%) within tumor tissues from WT ( n = 6) or KO ( n = 6) mice a and b. , and the CD40 + versus CD40 − MDSC subsets within WT tumor tissues ( c and d. n = 9). a) Representative flow cytometry images demonstrating the gating strategy and a higher MDSC% in tumor tissues from WT mice when compared with KO mice. b) Quantification of the MDSC% determined in a. c) Representative flow cytometry images demonstrating the gating strategy and a significantly higher percentage of CD40 + than CD40 − MDSC in WT tumor tissue. d) Quantification of the flow cytometry data shown in c.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Flow Cytometry

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: a. MFC were injected into WT ( n = 6) and KO ( n = 6) mice. Tumor growth was measured and is presented as tumor size at the indicated post-MFC inoculation time points. * p < 0.05, compared with tumors from CD40 KO mice. b. Fifteen days after MFC inoculation, the mice were sacrificed and tumors were excised and photographed. Tumors from WT mice were larger than tumors from KO mice. WT tumors were also irregularly shaped, with rough surfaces and ulcers.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Injection

Journal: Journal of neuroimmunology

Article Title: Role of CD40 in Prion Disease and the Immune Response to Recombinant PrP

doi: 10.1016/j.jneuroim.2013.01.008

Figure Lengend Snippet: Table 1

Article Snippet: The CD40 knockout mice (CD40 −/− ) were originally generated by Kawabe et al (1994) , and obtained commercially (CNCr.129P2- Cd40 tm1Kik /J, Jackson Labs) on the Balb/c background.

Techniques: Infection